Chemical and physicochemical mutagenization as a factor determinating the phenotype changes of selected biochemical features of Komagataeibacter xylinus bacteria
Justyna Płoska 1
Lidia Stasiak-Różańska 2
1 Slovak Academy of Sciences, Institute of Chemistry, Bratislava, Slovak Republic 2 Warsaw University of Life Sciences, Institute of Food Science, Warsaw, Poland
|Section:||Biotechnology and Food Technology|
The study used the Komagataeibacter xylinus strain belonging to the Acetic Acid Bacteria (AAB). As is know, AAB have been around mankind for many centuries. One of its most characteristic features is the ability to oxidize ethanol to acetic acid. In addition, these microorganisms have high metabolic potential, as a result of which they are producers of such compounds as bacterial cellulose, gluconic acid and dihydroxyacetone. These metabolites are used in various industries.
The aim of this research was to check the effect of chemical and physicochemical mutagenization on the production of these products by Komagataeibacter xylinus. At the beginning, a reproductive culture of K. xylinus was prepared, which was then subjected to chemical mutagenization using methyl methanesulfonate (MMS). Physicochemical mutagenization with the use of MMS and UV radiation was also used in the research. Selection to increase the production of gluconic acid, bacterial cellulose (BC) and dihydroxyacetone (DHA) as compared to the wild strain was made. Only the selection for increased BC production was successful. In the cases of gluconic acid and DHA no mutant with increased production capacity for this metabolites was obtained.
The study showed that chemical and physicochemical mutagenization methods may be effective in obtaining bacteria of the Komagataeibacter xylinus species with changed phenotypic characteristics. Also proved that technical and waste glycerol can be used as effective carbon sources in obtaining bacterial cellulose.