Design of in vitro model of oxidative damage of 3T3 cell line

Authors: Dominika Topoľská 1    Katarína Valachová 1    Ladislav Šoltés 1   
1 Ústav experimentálnej farmakológie a toxikológie SAV, Bratislava, Slovenska republika   
Year: 2015
Section: Cellular metabolism, physiology, molecular biology and genetics
Abstract No.: 1195
ISBN: 978-80-970712-8-8

Oxidative stress is associated with a wide variety of pathologies. An adequate model of oxidative damage of cells is important for the research of the effects of substances on such damaged cells.

Fibroblast is a basic cell of connective tissue, which plays a critical role in wound healing. 3T3 cells are a standard fibroblast cell line obtained from Swiss albino mouse embryo tissue.

The aim of this study was to design and development of in vitro model of oxidative damage of 3T3 cell line. As a relevant pathophysiological source of ROS components of the Weisberger biogenic oxidative system [Cu(II), ascorbate] were selected (Baňasová et al., 2014). Indication of ROS presence was performed by using flurescent dyes (dichlorofluorescin diacetate and dihydroethidium).

This work was supported by grant VEGA 2/0065/15.

M. Baňasová, K. Valachová, J. Rychlý, I. Janigová, K. Csomorová, R. Mendichi, D. Mislovičová, I. Juránek, L. Šoltés (2014) Effect of Bucillamine on Free-Radical-Mediated Degradation of High-Molar-Mass Hyaluronan Induced in vitro by Ascorbic Acid and Cu(II) Ions. Polymers 6, 2625-2644; doi:10.3390/polym6102625.